LAMP-PCR targeting HSP70 for screening Chagas disease v1

Sneider Alexander Gutierrez Guarnizo; Jessy Condori; Shirley Equilia; Edith Málaga; Jean Karla Velarde; Luciana Basma

doi:10.17504/protocols.io.e6nvwbpqzvmk/v1

October 26, 2024

LAMP-PCR targeting HSP70 for screening Chagas disease v1

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Key Points

LAMP-PCR assay effectively identifies multiple lineages of T. cruzi, demonstrating its reliability in screenings.
The assay targets the HSP70 gene of T. cruzi, facilitating specific identification without false positives.
This method is an isothermal amplification technique, offering rapid results and ease of use compared to traditional methods.
Results suggest LAMP-PCR could significantly improve Chagas disease diagnosis, allowing timely treatments and interventions.

Implication

LAMP-PCR identifies multiple T. cruzi lineages without cross-reacting with T. rangeli and Leishmania spp., indicating effective screening for Chagas disease.

Abstract

The Loop-mediated isothermal amplification LAMP-PCRTcHSP70 assay described here targets the HSP70 gene of T. cruzi (Gene ID: TcCLB.511211.170). It has been designed to identify multiple T. cruzi lineages without cross-reacting with T. rangeli or Leishmania spp.

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Cite This Study

Guarnizo et al. (2024) studied this question.

synapsesocial.com/papers/68af659bad7bf08b1eae58a6 https://doi.org/10.17504/protocols.io.e6nvwbpqzvmk/v1

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LAMP-PCR targeting HSP70 for screening Chagas disease v1

Key Points

Implication

Abstract

Discussion

Cite This Study

LAMP-PCR targeting HSP70 for screening Chagas disease v1

Key Points

Implication

Abstract

Discussion

Cite This Study

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