Detection of Trypanosoma cruzi with the Polymerase Chain Reaction and in Situ Hybridization in Infected Murine Cardiac Tissue
Population
Infected murine cardiac tissue sections
Design
Preclinical
Authors
Discussion
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Member takes
Implication
Caution against clinical adoption for chagasic cardiomyopathy diagnosis; leaves open translation from murine models to humans.
Structured PICO
P
Population
Infected murine cardiac tissue sections
I
Intervention
Polymerase chain reaction (PCR) amplification and in situ hybridization using three T. cruzi-specific DNA sequences (MCS, RS, FPS)
O
Outcome
Detection of T. cruzi amastigotes and DNA equivalentssurrogate
PCR and in situ hybridization effectively detect T. cruzi in murine cardiac tissue, indicating their potential utility for diagnosing human chagasic cardiomyopathy.
Cite This Study
Lane et al. (1997) studied this question.