Intracellular metabolism of peripherial blood leukocytes in arterial hypertension: experimental study

Objective: to conduct a comparative analysis of the features of intracellular metabolism in polymorphonuclear leukocytes (PMN) in the peripheral blood of rats with inherited stress-induced arterial hypertension (ISIAH) vs. normotensive animals (NTA). Materials and Methods. In the leukocytes of rat blood (15ISIAH rats and 20 NTA), the indicators of carbohydrate and lipid metabolisms, the activity of a number of key enzymes reflecting the state of redox processes, and PMN functional activity level were determined using histochemical methods. Results. The study established that a stable increase in blood pressure was accompanied by hypertrophy of the left ventricle, degenerative changes in cardiomyocytes and a decrease in the density of the microvasculature. At the same time, the amount of intracellular glycogen in leukocytes decreased by 23% while the amount of intracellular lipids remained unchanged. The levels of activity of intracellular ATPase and myeloperoxidase decreased by 22% with a slight increase in the activity of succinate dehydrogenase (by 13%). The number of PMN with a positive hematocrit (НСТ) test was higher by 35% in ISIAH rats.Conclusion. Analysis of the studied PMN metabolism indicators revealeddisorders in the energysupplying and enzymatic processes of leukocytes in arterial hypertension.