This method extracts RNA from saccharomyces cerevisiae using TRIzol and bead-beating, ensuring purity for RT-qPCR and RNA-seq.
Saccharomyces cerevisiae yeast cells were harvested by centrifugation and preserved in TRIzol reagent at −80 °C. Cell lysis was achieved via bead-beating in the presence of zirconium oxide beads using a high-speed homogenizer (Omni Bead Ruptor 12), resulting in >85% lysis after a single 30-second cycle, as verified by phase-contrast microscopy. RNA was extracted via chloroform-mediated phase separation and purified using the Ambion RiboPure™ Yeast RNA Purification Kit. This protocol provides an efficient method for isolating purified RNA suitable for downstream applications such as RT-qPCR and RNA-seq.
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Honey et al. (2025) studied this question.
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