Protocol enables high-quality RNA extraction from complex dough, enhancing transcriptomic analyses of yeast activity, suggesting improved workflows.
Bread dough is rich in polysaccharides and polyphenols, which hinder RNA extraction. We therefore established a streamlined workflow combining Fruit-mate homogenization, clarification, TRIzol extraction, high-salt precipitation, and activated-charcoal cleanup. This protocol reproducibly yields intact RNA of sufficient purity, enabling transcriptomic analyses of yeast activity in complex dough environments.
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Nishimura et al. (2025) studied this question.
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