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September 5, 2025Frontiers in Cellular and Infection MicrobiologyOpen Access

Rapid and accurate detection method for bluetongue virus based on CRISPR-Cas13a combined with RT-ERA

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Authors

DZDong ZhouHarbin Veterinary Research InstituteHYHaibo YuJiangnan UniversityYSYi ShaoAcademy of Medical Sciences

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Implication

This method reveals 100% clinical sensitivity for bluetongue virus detection in field samples, indicating its practical use in agriculture.

Key Points

  • The assay achieved a sensitivity of 20 RNA copies/reaction within 55 minutes, enhancing rapid detection.
  • Clinical validation with 263 samples resulted in 100% sensitivity across fluorescence modalities, demonstrating accuracy.
  • The RT-ERA/CRISPR-Cas13a platform is the first of its kind for pan-serotype bluetongue virus detection, emphasizing its advancements.
  • Field adaptation with Nucleic Acid Release Reagent maintained high clinical sensitivity, indicating robust field applicability.

Cite This Study

Zhou et al. (2025) studied this question.

synapsesocial.com/papers/68bb49c46d6d5674bccff7ddhttps://doi.org/10.3389/fcimb.2025.1621012
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Also Consider

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  1. 1Full-Genome Sequence of Bluetongue Virus Serotype 1 (BTV-1) Strain Y863, the First BTV-1 Isolate of Eastern Origin Found in China2013 · 16 citations
  2. 2HOLMESv2: A CRISPR-Cas12b-Assisted Platform for Nucleic Acid Detection and DNA Methylation Quantitation2019 · 805 citations
  3. 3Field-deployable viral diagnostics using CRISPR-Cas132018 · 1,521 citations
  4. 4Phylogenetic Characterization Genome Segment 2 of Bluetongue Virus Strains Belonging to Serotypes 5, 7 and 24 Isolated for the First Time in China During 2012 to 20142016 · 33 citations
  5. 5Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm62018 · 2,758 citations