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This is a Experimental Study study.

October 3, 2025PeerJOpen Access

Development and application of a CRISPR/Cas12a-based reverse transcription–recombinase polymerase amplification assay with lateral flow dipstick and fluorescence detection for Getah virus

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This assay achieves high sensitivity with 10 copies/µL detection of Getah virus, and demonstrates 100% concordance with PCR results.
Using a multiplex detection method, the optimized setup enables quick visualization via lateral flow dipstick or fluorescence detection.
Analysis showed exclusive specificity against other pathogens, enhancing reliability in diagnosing Getah virus and aiding public health measures.
The method's simplicity allows for practical deployment in field settings, essential for timely detection and containment of Getah virus infections.

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Implication

This research develops a sensitive diagnostic method combining CRISPR/Cas12a with RT-RPA for Getah virus detection, indicating significant implications for public health.

Cite This Study

Xia et al. (2025) studied this question.

synapsesocial.com/papers/68e040f7a99c246f578b3aca https://doi.org/10.7717/peerj.20119

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Implication

This research develops a sensitive diagnostic method combining CRISPR/Cas12a with RT-RPA for Getah virus detection, indicating significant implications for public health.

Development and application of a CRISPR/Cas12a-based reverse transcription–recombinase polymerase amplification assay with lateral flow dipstick and fluorescence detection for Getah virus

Key Points

Discussion

Implication

Cite This Study

Development and application of a CRISPR/Cas12a-based reverse transcription–recombinase polymerase amplification assay with lateral flow dipstick and fluorescence detection for Getah virus

Key Points

Discussion

Implication

Cite This Study

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