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April 2, 2025BMC Veterinary ResearchOpen Access

Rapid and visual detection of transmissible gastroenteritis virus using a CRISPR/Cas12a system combined with loop-mediated isothermal amplification

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Why the study?

Existing detection methods for transmissible gastroenteritis virus are complex, demanding specialized tools and significant time, necessitating a precise, swift, and effective diagnostic method.

Population

Transmissible gastroenteritis virus targeting the N gene

Design

In vitro diagnostic development study

Authors

HWHaiyang WangQZQi ZhaoJWJiale Wang

Discussion

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Overview

Offers higher-sensitivity TGEV detection in animal models; leaves open clinical translation pending field validation.

Structured PICO

P
Population
In vitro assay targeting the N gene of transmissible gastroenteritis virus (TGEV)
I
Intervention
CRISPR-Cas12a fluorescence assay and CRISPR/Cas12a lateral-flow dipstick assay combined with loop-mediated isothermal amplification (LAMP)
O
Outcome
Assay sensitivity and cross-reactivitysurrogate

A novel CRISPR-Cas12a combined with LAMP assay provides a highly sensitive and specific method for rapid visual detection of TGEV.

Cite This Study

Wang et al. (2025) studied this question.

synapsesocial.com/papers/6a82582df4cd2cf3fab4a94ahttps://doi.org/10.1186/s12917-025-04711-1
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Detection and phylogenetic analyses of spike genes in porcine epidemic diarrhea virus strains circulating in China in 2016–20172017 · 31 citations
  2. 2The transmissible gastroenteritis coronavirus contains a spherical core shell consisting of M and N proteins1996 · 153 citations
  3. 3APB‐13 improves the adverse outcomes caused by TGEV infection by correcting the intestinal microbial disorders in piglets2021 · 9 citations
  4. 4CRISPR/Cas12a Technology Combined With RPA for Rapid and Portable SFTSV Detection2022 · 58 citations
  5. 5Visual and Rapid Detection of Porcine Epidemic Diarrhea Virus (PEDV) Using Reverse Transcription Loop-Mediated Isothermal Amplification Method2022 · 24 citations