A taxonomic revision of Aureobasidium (Saccotheciaceae, Dothideales) with new species, new names, and typifications

Fig. S1A. Phylogenetic inference of ITS sequences of 208 Aureobasidium isolates. The phylogram is based on Bayesian inference (BI) and maximum likelihood (ML) analyses. The bootstrap percentages of the ML ≥ 70 % and BI posterior probability values ≥ 0.90 are presented at the nodes (as ML/BI). Values < 70 % bootstrap support or < 0.90 posterior probability are indicated with a hyphen or not shown. The scale bar indicates the number of nucleotide substitutions per site. Sequences from ex-types are indicated with an asterisk (*). Bold = new names from this study. The red star represents the A. pullulans species complex sensu lato. The five groups also identified in the single locus phylogenies are indicated as PullG, NamiG, MelaG, SubgG and ThaiG (see text for explanation). Fig. S1B. Phylogenetic inference of 28S sequences of 103 Aureobasidium isolates. The phylogram is based on Bayesian inference (BI) and maximum likelihood (ML) analysis. The bootstrap percentages of the ML ≥ 70 % and BI posterior probability (pp) values ≥ 0.90 are presented at the nodes (as ML/ BI). Values < 70 % bootstrap support or < 0.90 posterior probability are indicated with a hyphen or not shown. Vertical bars indicate the different species groups delineated as explained in the text. The scale bar indicates the number of nucleotide substitutions per site. The ex-types are indicated with an asterisk (*). Fig. S1C. Phylogenetic inference of EF1a gene sequence of 50 Aureobasidium isolates. The phylogram is based on Bayesian inference (BI) and maximum likelihood (ML) analysis. The bootstrap percentages of the ML ≥ 70 % and BI posterior probability values ≥ 0.90 are presented at the nodes (as ML/BI). Values < 70 % bootstrap support or < 0.90 posterior probability are indicated with a hyphen or not shown. Vertical bars indicate the different species groups delineated as explained in the text. Scale bar indicates the number of nucleotide substitutions per site. The tree is rooted with Delphinella strobiligena (AFTOL-ID 1257) and Sydowia polyspora (AFTOL-ID 1300). The ex-types are indicated with an asterisk (*). Fig. S1D. Phylogenetic inference of ELO2 sequence of 50 Aureobasidium isolates. The phylogram is based on Bayesian inference (BI) and maximum likelihood (ML) analysis. The bootstrap percentages of the ML ≥ 70 % and BI posterior probability (pp) values ≥ 0.90 are presented at the nodes (as ML/ BI). Values < 70 % bootstrap support or < 0.90 posterior probability are indicated with a hyphen or not shown. Vertical bars indicate the different species groups delineated as explained in the text. Scale bar indicates the number of nucleotide substitutions per site. The tree is rooted with Delphinella strobiligena (AFTOL-ID 1257) and Sydowia polyspora (AFTOL-ID 1300). The ex-types are indicated with an asterisk (*). Fig. S1E. Phylogeny inferred from the mtLSU gene sequence of 35 Aureobasidium isolates. The phylogram is based on Bayesian inference (BI) and maximum likelihood (ML) analysis. The bootstrap percentages of the ML ≥ 70 % and BI posterior probability (pp) values ≥ 0.90 are presented at the nodes (as ML/BI). Values < 70 % bootstrap support or < 0.90 posterior probability are indicated with a hyphen or not shown. Vertical bars indicate the different species groups delineated as explained in the text. The bar indicates the number of nucleotide substitutions per site. The tree is midpoint rooted. The ex-types are indicated with an asterisk (*). Fig. S1F. Phylogeny inferred from the mtSSU gene sequence of 24 Aureobasidium isolates. The phylogram is based on Bayesian inference (BI) and maximum likelihood (ML) analysis. The bootstrap percentages of the ML ≥ 70 % and BI posterior probability (pp) values ≥ 0.90 are presented at the nodes (as ML/BI). Values < 70 % bootstrap support or < 0.90 posterior probability are indicated with a hyphen or not shown. Vertical bars indicate the different species groups delineated as explained in the text. The bar indicates the number of nucleotide substitutions per site. The tree is midpoint rooted. The ex-types are indicated with an asterisk (*). Fig. S1G. Phylogeny inferred from the RPB2 gene sequence of 82 Aureobasidium isolates. The phylogram is based on Bayesian inference (BI) and maximum likelihood (ML) analysis. The bootstrap percentages of the ML ≥ 70 % and BI posterior probability (pp) values ≥ 0.90 are presented at the nodes (as ML/BI). Values < 70 % bootstrap support or < 0.90 posterior probability are indicated with a hyphen or not shown. Vertical bars indicate the different species groups delineated as explained in the text. The bar indicates the number of nucleotide substitutions per site. The tree is midpoint rooted. The ex-types are indicated with an asterisk (*). Fig. S1H. Phylogeny inferred from the TUB gene sequence of 37 Aureobasidium isolates. The phylogram is based on Bayesian inference (BI) and maximum likelihood (ML) analysis. The bootstrap percentages of the ML ≥ 70 % and BI posterior probability (pp) values ≥ 0.90 are presented at the nodes (as ML/BI). Values < 70 % bootstrap support or < 0.90 posterior probability are indicated with a hyphen or not shown. Vertical bars indicate the different species groups delineated as explained in the text. The bar indicates the number of nucleotide substitutions per site. The tree is rooted with Lecythophora hoffmannii (CBS 245.38). The ex-types are indicated with an asterisk (*). Fig. S1I. GHOST phylogeny inferred from the concatenated dataset with linked GTR parameters. We used the GTR+FO+H4 rate substitution mthe overall maximum likelihood phylogeny in Fig. 1A. The values above branches represent node ages multiplied by 100. The numbers in square brackets are the convergence frequencies of the branches. Table S1. Sequenced strains and accession numbers of Aureobasidium isolates analysed in this study, including the geographic origin and substrate from which the strains were isolated. Table S2. Data on colony diam. (mm) and assimilation/fermentation profiles of three additional strains of new Aureobasidium species described. Plates for colony diam. were incubated at 25 °C unless otherwise noted.