Figure 1 from GALNT7 Stratifies dMMR/MSI Colorectal Cancer into Distinct Molecular Subsets Associated with Prognosis and PD-L1 Expression

Integration of bulk, cell line, and single-cell RNA-seq for 188 glycosyltransferase genes identified five genes that were differentially expressed between MSI and MSS colorectal cancers. A, Heatmap depicting the upregulation of GALNT7, GALNT1, and HPSE and the downregulation of GALNT6 and ST6GAL1 in MSI colorectal cancer bulk tissues (TCGA cohort), cell lines (CCLE cohort), and single epithelial cancer cells (SMC cohort), compared with those of MSS. Dot size corresponds to the percentage of cells expressing the gene. B, Heatmap depicting the performance of Glyco-MSI score based on the expression of five genes to distinguish MSI from MSS colorectal cancers using 19 transcriptomic and proteomic cohorts of colorectal cancer. C, Forest plot demonstrating Cox HRs and 95% CIs of each five gene and the Glyco-MSI score for OS in TCGA cohort, PFS in the AC-ICAM cohort, and RFS in the GSE39582 cohort. D–F, Kaplan–Meier curves according to the expression of GALNT7 in TCGA (D), AC-ICAM (E), and GSE39582 (F). Log-rank P values, Cox HRs, and 95% CIs are indicated. For survival analysis, patients were dichotomized as high or low based on the median expression of GALNT7.