Exploring the Therapeutic Potential: Investigating the Synthesis, Characterization and in vitro Anti-Diabetic Efficacy of Silver Nanoparticle Ethanolic Extract from Boerhavia diffusa

Many studies explore the effects of Boerhavia diffusa (Bd) combined with synthesized silver-nanoparticles extract (AgNPs) for treating a variety of conditions, although rarely for diabetes. To address this, we investigate the inhibitory effect of BdAgNPs in standard in vitro antidiabetic assays using the whole plant extract dissolved in ethanol (EBdAgNPs). EBdAgNPs was characterized by UV-Visible, XRD and FT-IR spectroscopy techniques. The assays consisted of α-amylase, α-glucosidase, protein glycation, non-enzymatic glycosylation of hemoglobin, glucose uptake by yeast and glucose diffusion. The formation of AgNPs was revealed by observing a surface Plasmon resonance peak at 410 nm. Notably, EBdAgNPs exhibited robust inhibitory effects in comparison to standard control drugs within the assays. These compelling findings strongly indicate the potential of EBdAgNPs as a viable avenue for treating diabetes mellitus. Further clinical investigations are warranted to fully explore EBdAgNPs potential in novel therapeutic approaches for managing diabetes and related conditions.