Anti-hyperglycaemic effect of aqueous extract from

Brosimum alicastrum bark is used in Mexican traditional medicine to treat chronic illnesses, including diabetes. For this reason, the purpose of this work was to evaluate the anti-hyperglycaemic effect of the aqueous extract from B. alicastrum bark. Therefore, the aqueous extract at 40 mg/kg, administered orally and chronically, exhibited an anti-hyperglycaemic effect in a streptozotocin/nicotinamide model in Wistar rats. Because of our chemical study of this species demonstrated the presence of the coumarins xanthyletin (1), luvangetin (2), 8-hydroxyxanthyletin (3), and methoxyrutaretin (4), we also determined the presence of this bioactive compounds in the aqueous infusion and their quantification therein by developing a new, simple, fast, selective, sensitive, and precise HPLC-DAD method according to ICH validation guideline. Finally, the presence of coumarins can be associated with the anti-hyperglycaemic effect shown by the extract.