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Challenges and limitations of molecular resolution fluorescence imaging

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Molecular resolution imaging in cells remains a significant challenge with super-resolution microscopy.
Many developed SRM methods achieve nanometer precision, yet fail under physiological conditions.
Advances in spatial resolution push the boundaries of imaging but highlight substantial practical difficulties.
The application of these methods in living cells is limited despite technological progress in fluorescence imaging.

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Super-resolution microscopy struggles to achieve molecular resolution imaging in cells, highlighting significant limitations.

Helmerich et al. (2025) studied this question.

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