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September 10, 2025

Enzymatic Ethanol Assay v2

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Authors

SHSarah K. HammerDKDev KapadiaSHShuen Hon

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Overview

This enzymatic assay estimates ethanol concentrations in samples, highlighting rapid detection and low cost.

Key Points

  • The enzymatic assay reliably estimates ethanol concentrations within one hour.
  • Using alcohol dehydrogenase, the assay converts ethanol into acetaldehyde while monitoring NADH production.
  • This assay offers a low-cost alternative to high performance liquid chromatography for ethanol detection.
  • The method is applicable for monitoring ethanol production in microbial fermentations and evaporation from media.

Cite This Study

Hammer et al. (2021) studied this question.

synapsesocial.com/papers/68c19ab49b7b07f3a061c97ahttps://doi.org/10.17504/protocols.io.bp2l6b8n5gqe/v3
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