In Vitro Evaluation of Anti-inflammatory and Anti-Cancer Activities of Flower Extracts of Abelmoschus esculentus L.

Oxidative stress and inflammation are the predominant cause of chronic diseases, including multiple forms of cancers. Prevention of oxidative stress and inflammation is considered to be a target for preventing these disorders due to their significant roles in various degenerative diseases. Various natural products and plant extracts prevent the process of free radical- induced damages. The present study evaluated the biological properties of Abelmoschus esculentus L, which is a traditionally used Ayurvedic plant. Protein denaturation inhibition bioassay and Proteinase Inhibition assays were used for anti-inflammatory studies. Anticancer activity was evaluated using Brine shrimp lethality bioassay. The results of the present study anti-inflammatory properties were also evident in terms of Protein denaturation inhibition bioassay the Hydroalcoholic extract's IC50 value is 166.56 µg/ml, ethanolic extract's is 220.80 µg/ml, aqueous extract IC50 value is 234.01and Proteinase Inhibition assay the IC50 values for Hydroalcoholic, ethanolic and aqueous extracts were 131.54, 150.99 and 221.84 µg/mL, respectively. The benefit of a lethal dose (LD50) is that it can be used to reduce significantly the number of animals used.(37) In Abelmoschus esculentus L the hydroalcoholic extract showed the lowest LC50 value (152.90 µg/ml) and in ethanol showed lowest LC50 Value (241.58µg/ml). According to the definition of LC50, the high LC50 value means it has less toxicity and low LC50 value means it has more toxicity that means, hydroalcoholic extract from Abelmoschus esculentus L contained more toxic compounds (low LC50 value means high toxic). The study thus concludes that Abelmoschus esculentus L showed significant anti-inflammatory and anticancer properties. Further studies together with a bioassay-guided fractionation may identify possible bioactive compounds.