Determination of the sensitizing activity of a gel with octenidine dihydrochloride

In modern animal husbandry, a large proportion of diseases of non-infectious etiology is occupied by animal injury, which leads to damage to the skin. We have developed a new antiseptic wound healing drug, which includes octenidine dihydrochloride, pantothenic acid and auxiliary components that potentiate the action of the main components of the drug. The study of sensitizing activity included two studies - the method of skin applications and a conjunctival test. It was conducted in accordance with the "Guidelines for conducting preclinical studies of medicines" edited by A.N. Mironov (2012), GOST 32375-2013 "Testing methods for the effects of chemical products on the human body. Skin sensitization assessment tests". The skin sensitization test included the determination of induction and provocative exposure, and was conducted on guinea pigs. Two groups of animals were formed – the study group and the control group. There were 20 animals in the study group and 10 in the control group. The second part of the study of the sensitizing activity of the drug is a conjunctival test. Guinea pigs were used for the study. Two groups of animals were formed – the study group and the control group, each with 10 animals. When assessing skin sensitization, the following results were obtained: redness, local increase in skin temperature, itching, thickening of the skin fold, peeling of the skin – were absent. There were no systemic changes, and the behavior of the animals did not change throughout the study. When examining a conjunctival sample, a gel with octenidine dihydrochloride caused redness in the area of the tear duct, cornea and sclera 15 minutes after administration of the drug under the upper eyelid. After 24 and 48 hours, there was no redness. There was no itching during each observation stage. There were no changes in the control group. No systemic changes were detected in both groups during the entire study period.