Expression of antioxidant protection and immunity genes in broiler chicken tissues under the influence of a complex of adaptogens

Modern poultry farming technologies are often associated with stress factors that disrupt the balance between free radical production and antioxidant defense, potentially leading to oxidative stress. Consequently, maintaining optimal immune system function and enhancing antioxidant defense, particularly through exogenous antioxidants, is critical for poultry health and adaptation. This study aimed to evaluate the impact of the DHQE С complex (dihydroquercetin, vitamins E and C) on the expression of antioxidant and immune-related genes in the liver and intestinal tissues of broiler chickens under simulated stress. The experiment was carried out on broiler chickens of the «Smena-9» cross (n=40, N=160). A control group and three experimental groups were formed, which were subjected to stress (increase in stocking density by 10%). In addition to the basal diet, the third (from the 21st day of the experiment) and fourth (from the 1st day of the experiment) groups were supplemented with DHQEC : dihydroquercetin (32 mg/kg feed), vitamin E (10 mg/kg feed), and vitamin C (35 mg/kg feed). Realtime PCR was used to analyze the expression of Nrf2, GSH-Gpx, CAT, SOD, HO-1, AvBD9, IL-6, and IL-8 genes in liver and cecum. Results demonstrated that simulated stress reduced the expression of Nrf2 and GSH - Gpx in intestinal and liver tissues, along with decreased AvBD9 expression, indicating suppressed defense mechanisms. Prophylactic DHQEС administration mitigated these effects by upregulating Nrf2, GSH-Gpx , and HO-1 expression, while stimulating AvBD9 and IL-6. A positive correlation was observed between intestinal AvBD9 expression and average daily weight gain. These findings highlight the potential of the DHQE С complex to enhance stress resilience and productivity in broilers.