Adaptation of the Prodoxin-aflatoxin B1 ELISA kit to determine the concentration of Aflatoxin B1 in pig organs

Mycotoxins are a group of biologically active substances, the penetration of which into the organism of farm animals cannot be completely prevented. The article presents the results of experimental studies on the adaptation of the PRODOSCREEN IFAAflatoxin B 1 kit for determining the concentration of aflatoxin B 1 in pig liver homogenates. The material (whole liver) was collected from one animal at a slaughterhouse. During sample preparation, the liver was homogenized and randomly divided into 5- gram portions. The technique is based on the formulation of a direct competitive enzyme immunoassay, the principle of which is the selective binding of a specific antigen to an antibody, which, under the action of an enzyme, as part of an antibody-bound conjugate, turns into a colored product. The essence of the set of measures for the adaptation of this method is to use the technique for searching for antigens not in the plant materials declared in the instructions, but in the organs of pigs. The article provides detailed data on the preparation of organ samples for the study, calculations of aflatoxin B 1 dosages introduced into the liver homogenate, and describes the stages of setting up an enzyme immunoassay. The data obtained during the experiment on the adaptation of the ELISA kit for determining the concentration of aflatoxin B 1 not only in plant materials, but also in pig liver homogenates, allow us to draw a number of conclusions where direct competitive ELISA can be used as part of a comprehensive diagnosis of mycotoxicoses in agricultural enterprises as an express method for qualitative (aflatoxin B 1 is in the organ / aflatoxin B 1 is not in the organ) assessment of the presence of aflatoxin B 1 in pig organs. Also, taking into account the measurement error from 0 to 28%, this method allows for a semi-quantitative assessment of the content of aflatoxin B1 in pig liver homogenate. We also noted that the lower the volume of Aflatoxin B 1 introduced into the liver homogenate, the lower the error in determining these concentrations using the direct competitive ELISA method.