Synapse
⌘+K
Synapse
PulseExploreClubsResearchersJournals
Instagram
HomeClubsExplore
September 10, 2025Light Science & ApplicationsOpen Access

Wide-field fluorescence lifetime imaging of single molecules with a gated single-photon camera

View Full Paper
Ask AI
Bookmark
Share

Authors

NRNathan RonceraySBSalim BennaniMMMarianna Fanouria Mitsioni

Discussion

Loading...

Member takes

Overview

This research demonstrates the effectiveness of a time-gated camera for high-throughput single-molecule fluorescence lifetime imaging, indicating new possibilities for protein analysis.

Key Points

  • Accurate single-molecule lifetime measurements were achieved, with precision only three times lower than TCSPC's.
  • Fast imaging of over 3000 molecules was performed in a 512 × 512 pixel array, highlighting data-rich parallelization capabilities.
  • Temporal single-molecule Förster resonance energy transfer was measured at frequencies of 5-25 Hz, showing detailed dynamic interactions.
  • This method shows significant potential for advancing multi-target single-molecule techniques and biopolymer sequencing.

Cite This Study

Ronceray et al. (2025) studied this question.

synapsesocial.com/papers/68c1ac0954b1d3bfb60e48f3https://doi.org/10.1038/s41377-025-01901-2
View Full Paper
Ask AI
Bookmark
Share