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September 10, 2025Animal GeneticsOpen Access

Development of bovine leukocyte antigen – DRB3 genotyping using nanopore sequencing

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Authors

TAToshiro AraiYKYukiko KanetsunaSMSatoshi Miyaké

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Overview

This method demonstrates high accuracy in genotyping BoLA-DRB3 in cattle, resolving phase ambiguity effectively.

Key Points

  • Nanopore sequencing achieved 99.97% coverage for BoLA-DRB3 in cattle samples, improving efficiency.
  • The method correctly aligned reference sequences in 92.7% of 109 samples, validating its accuracy.
  • Using blood samples from Japanese Black cattle, the technique reduced processing time from days to hours.
  • This new approach offers a promising standard for genotyping bovine MHC genes, addressing PCR-SBT limitations.

Cite This Study

Arai et al. (2025) studied this question.

synapsesocial.com/papers/68c1ac0954b1d3bfb60e4c6bhttps://doi.org/10.1111/age.70033
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