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September 10, 2025Afyon Kocatepe University Journal of Sciences and EngineeringOpen Access

Investigation of the Usability of Polymeric Nanoparticle-Based Transfection Agent for CRISPR/CAS9 in HUVEC Cell Line

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Authors

RİRızvan İmamoğluÖKÖzlem KaplanMGMehmet Koray Gök

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Overview

Investigation reveals effective gene silencing in HUVEC cells using a polymeric transfection agent, indicating CRISPR's potential.

Key Points

  • The study found that a polymeric nanoparticle transfection agent achieved 90-95% efficiency in HUVEC cells.
  • CRISPR efficiency for gene silencing of COX-2 was confirmed at 57.12% through Western blot analysis.
  • HUVEC cells demonstrated effective transfection, showing potential for CRISPR-based therapeutic applications.
  • The findings support the use of PβAE nanoparticles in enhancing CRISPR delivery for gene therapies.

Cite This Study

İmamoğlu et al. (2025) studied this question.

synapsesocial.com/papers/68c1ad5554b1d3bfb60e5237https://doi.org/10.35414/akufemubid.1584459
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