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December 10, 2025Langmuir

Core–Shell Tripeptide-Lipid/PEI Nanocarriers Enable Efficient Plasmid-Based CRISPR/Cas9 Editing of VEGFR2

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Authors

ZGZhao GaoKLKexin LinYGYan Gong

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Overview

Delivery of CRISPR/Cas9 via lipid nanoparticles improved genome editing in cancer models, indicating potential for gene therapy.

Key Points

  • The study aims to enhance CRISPR/Cas9 delivery for efficient genome editing targeting VEGFR2 in cancer cells.
  • Constructed ternary systems using pDNA, polyethylenimine (PEI), and lipid nanoparticles
  • Analyzed transfection efficiency in A549 and MCF-7 cell lines
  • Conducted Western blot to assess VEGFR2 and PI3K levels
  • Achieved transfection efficiencies up to 91% in A549 and MCF-7 cells
  • VEGFR2 expression decreased by approximately 48% in A549 and 44% in MCF-7 cells
  • Inhibition of the PI3K/Akt pathway led to cell cycle arrest and reduced tumor cell proliferation

Cite This Study

Gao et al. (2025) studied this question.

synapsesocial.com/papers/69401d472d562116f28f85b5https://doi.org/10.1021/acs.langmuir.5c02069
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