This protocol details a method to amplify poliovirus RNA using PCR, enabling whole-genome sequencing for surveillance and research.
This protocol describes the amplification and whole-genome sequencing of poliovirus from clinical or environmental RNA extracts using an overlapping tiled amplicon strategy. Four overlapping PCR products are generated from viral RNA via RT-PCR. Amplicons are pooled and purified before library preparation for Oxford Nanopore or Illumina sequencing. This protocol enables robust recovery of full poliovirus genomes for surveillance and research purposes.
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Arowolo et al. (2025) studied this question.
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