Using ISSRmarkers for genotyping Calligonum aphyllum (Pall.) Guerke, growing in the Astrakhan region and Stavropol region

To date, the issues of genotyping of various groups of woody and shrubby plants that grow in arid conditions remain relevant to determine breeding-valuable genotypes. In this regard, the purpose of the study was to test ISSR markers and use them for subsequent genotyping of various ecotopic populations of C. aphyllum native to the Astrakhan Region and Stavropol Territory. The effectiveness of the ISSR primers used was evaluated using four main parameters of the information content of primers: polymorphism information index (PIC), effective multiplexing coefficient (EMR), marker index (MI) and resolution (Rp). Using 9 primers, a total of 88 DNA fragments of the C. aphyllum genome were amplified, of which 77 were polymorphic. The total number of amplified DNA bands depended on the primer used and ranged from 8 to 15. The average PIC value using nine primers was 0.381, while the standard deviation was only 0.0043. The MI values ranged from 1,293 to 2,371, with an average of 1,723. The highest MI values were found in primers UBC808, UBC856 and UBC891 – 1,850, 1,908 and 2,371, respectively. The resolution index values ranged from 2,400 (UBC808) to 7,771 (UBC891). The average RP value was 4,508, which indicates the high efficiency of the primers studied in detecting genetic differences in C. aphyllum populations. Populations in the Astrakhan region have 8.2% higher polymorphism than populations in the Stavropol Territory. Both populations from the Astrakhan region (Cal2 and Cal3) show similar values of all parameters of genetic variability. The research results can be used in breeding work to create new forms of C. aphyllum.