Evaluation of Physico-chemical, Phytochemical Analysis, Invitro Antioxidant, Anti-inflammatory activity and FTIR Spectral analysis of Crude Extract from Jasminum grandiflorum Flower (Linn.)

Objective: Jasminum grandiflorum, which belongs to the Oleaceae family, is most commonly used in the fragrance industry due to its aromatic compound content.It is a medicinally significant plant widely used in traditional and Ayurvedic medicine. While its flowers and leaves are well known for their therapeutic benefits, the stem also contains bioactive compounds that contribute to its pharmacological properties.The current goal of the research is to usephysicochemical, qualitative and quantitative estimation of phytochemicals, determination of total phenol and flavonoid content, antioxidant activity, Anti-inflammatory and FT-IR spectral analysis in floral parts of Jasminum grandiflorum. Methods: The solvents methanol, and aqueous are used for extraction of Jasminum grandiflorum plant parts. Phytochemical screening of plant parts was carried out in both the solvents. Determination of total phenol content was carried out using Folin – Ciocalteau method and total flavonoid content using Aluminium chloride spectrophotometric method. Antioxidant activity of methanolic extract of plant samples were evaluated with DPPH standard method. The FT-IR is a very useful technique for identifying the functional groups present in the mixture. Results: The result revealed the presence of flavonoids, saponins, tannins, Steroidal glycosides, Triterpenoids, Carbohydrate , Phenolic compound, tannins steroids in methanol and aqueous extract but alkaloids were absent in aqueous extract. Total phenol content was expressed in mg of Gallic Acid Equivalent (GAE) per g of dry weight. In results it was found that methanol extract shows highest phenol content 465.94 mg/g in flowers. The content of flavonoids was expressed in mg of Quercetin Equivalent (QE) per g of dry weight. It was evaluated that total flavonoid content found highest in flowers 445.24 mg/g in methanol extract. IC50 for standard ascorbic acid was found to be 87.89 µg/ml and for methanol and aqueous extract flower was found to be 65.73 µg/ml and 69.73. µg/ml respectively. The DPPH radical scavenging activity of Jasminum grandiflorum was evaluated and compared with ascorbic acid. The presence inhibition of flowers extract was calculated at various concentration (10,20,30,40,50 etc.) as well as standard ascorbic acid. The highest scavenging activity of methanolic and aqueous extract were 47.1.9±0.207 % and 45.2±0.403 % at concentration of 50 µg/ml. FT-IR analysis of methanol flower and aqueous extracts of Jasminum grandiflorum confirmed the presence of phenols, alcohols, carboxylic acid, amide, aldehydes, ketones, alkanes, alkenes, aromatics, amines and alkyl halides which show major peaks. Conclusion: The results obtained from the preliminary standardization of Jasminum grandiflorumare very helpful in the determination of the quality and purity of the crude drug. The refurbished findings of Jasminum grandiflorumare promising, and further research is important to identify the bioactive compounds, thereby developing nutritional supplements and medications through therapeutic compound isolation.