This study reveals changes in nitrogen transformation in soil due to microbes, suggesting bacterial preparations enhance biogenicity.
The article examines the determination of the number of major groups of microorganisms, the characteristics of soil biogenicity and the effect on nitrogen transformation. The soils involved in agricultural production are subject to a number of negative factors due to the use of various kinds of chemicals, violation of agricultural technology of cultivated crops, non-compliance with crop rotations, etc. The alternative to solve these issues is the possibility of using preparations based on microorganisms in specific conditions of the region. The experiment took into account the number of ammonia microorganisms on nutrient media meat-peptone agar (MPA), amylolytic microflora growing on starch-ammonium agaric (SAA), the number of fungal microflora on Chapek medium on soil samples taken from field experience laid on gray forest heavy loamy soil, which serves as the basis for testing various basic surface tillage to a depth of 10-12 cm and landfill plowing to a depth of 20-22 cm, as well as the use of the biological fungicide Sternifag based on the Trichoderma fungus and a microbiological preparation based on the Biocomposite consortium of microorganisms. Based on the conducted studies, the coefficients of mineralization, nitrogen immobilization, and transformation in spring wheat and peas were calculated. The results obtained show an increase in biogenicity when using a bacterial preparation in the studied periods to 38.69 million. CFU/g in 2023 and up to 42.11 million. CFU/g in 2024. The change in the number of microorganisms from 22.1 million. CFU/g in 2023 to 18.42 million. CFU/ g in 2024 on MPA medium, the change in the number of microorganisms from 17.48 million. CFU/g in 2023 to 24.3 million. CFU/g in 2024 on the environment of KAA, bacteria from 31.40 million. CFU/g in 2023 to 25.51 million. CFU/g in 2024, actinomycetes from 10.05 million. CFU/g in 2024 compared to the number of 23.29 million. CFU/g in 2023, micromycetes from 66.67 million. CFU/g in 2023, up to 19.13 million. CFU/g in the conditions of 2024.
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Roman A. Goryaev (2025) studied this question.
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