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September 10, 2025Biological Procedures OnlineOpen Access

Refining Flow Cytometry-based Sorting of Plasma-derived Extracellular Vesicles

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This study demonstrates enhanced sorting accuracy of cancer-derived extracellular vesicles in plasma, suggesting improved biomarker applications.

High-purity sorting of CD9 + EVs supports identification of cancer biomarkers and develops targeted therapies.
Detection and sorting of EVs improved through optimized flow cytometry techniques and instrument calibration.
Plasma EVs isolated using sucrose cushion ultracentrifugation provided enhanced purity and yield for analyses.
Study highlights the importance of accurately characterizing EV subpopulations for understanding cancer progression and treatment response.

Reverberi et al. (2025) studied this question.

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This study demonstrates enhanced sorting accuracy of cancer-derived extracellular vesicles in plasma, suggesting improved biomarker applications.