This protocol outlines a method to extract soluble and insoluble protein fractions from mouse brains using Triton X-100 and SDS, enabling further biochemical analysis.
This protocol describes a sequential detergent-based fractionation method for mouse brain tissue. Homogenized samples are sonicated, with aliquots reserved as total lysate. Stepwise extraction with Triton X-100 and SDS yields soluble and insoluble protein fractions, while remaining pellets may be saved for formic acid extraction. Protein concentrations are determined by BCA assay, and fractions are stored at −80 °C for downstream biochemical analysis.
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Scholz et al. (2025) studied this question.
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