This protocol can be used to isolate protein from murine brain tissue (volumes listed are for hippocampal samples) by solubility. It creates three protein fractions: tris soluble, triton soluble, and formic acid soluble. This protocol can be helpful when evaluating amyloid beta pathology as different amyloid beta structures can be found within each fraction. For example, insoluble amyloid beta associated with amyloid plaques will be found within the formic acid soluble fraction, whereas more soluble non-plaque associated amyloid beta will be found within the other two fractions.
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Blackmer‐Raynolds et al. (2024) studied this question.
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