Synthesis, Characterization and Evaluation of Mucuna Pruriens Zinc Nanoparticles: Investigating Anti-Inflammatory, Antioxidant, and Anti-Arthritic Properties

Objectives: The objective of the study was to synthesize zinc (Zn) nanoparticles (NPs) using Mucuna pruriens extract and evaluate their antioxidant, anti-inflammatory, and anti-arthritic activities through in vitro assays Methods: Zn NPs were synthesized from M. pruriens and subjected to a series of in vitro biological assays, including 2,2-diphenyl-1-picrylhydrazyl radical scavenging (antioxidant), protein denaturation inhibition, anti-proteinase activity, heat-induced hemolysis, and human red blood cell (HRBC) membrane stabilization methods (anti-inflammatory and anti-arthritic evaluations). Comparisons were made with standard reference drugs Results: The M. pruriens-derived Zn NPs demonstrated significant antioxidant, anti-inflammatory, and anti-arthritic activities. They effectively inhibited protein denaturation and stabilized red blood cell membranes, often outperforming standard drugs in the tested parameters. Conclusion: M. pruriens-derived Zn NPs exhibit potent biological activities, highlighting their potential as a natural therapeutic strategy for managing oxidative stress and inflammatory diseases. Further in vitro and preclinical studies are recommended to confirm these findings and explore their clinical applicability.