September 11, 2025

MksEF Accessory Proteins Inhibit MksB ATPase Activity and Modulate DNA Substrate Binding.

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Experimental analysis shows that MksEF enhances DNA binding affinity of MksB, indicating a unique regulatory role.

MksB's DNA-binding ability is enhanced by accessory proteins MksE and MksF, promoting local chromosome organization.
The study identified the stoichiometry of the MksBEF complex as MksB2E4F2 using gel filtration and ultracentrifugation methods.
Gel shift assays demonstrated that MksEF lowers the ATPase activity of MksB, showcasing a distinctive regulatory mechanism.
Fluorescence experiments indicated structural rearrangements in MksB during DNA binding, contributing to our understanding of chromosomal dynamics.

Kumari et al. (2025) studied this question.

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