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September 14, 2025

High agreement between one-stage clot-based and chromogenic factor VIII activity assays.

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Authors

RBRadhiya Al BuraidiMMMuntadhar Al MoosawiKFKarima Al Falahi

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Overview

Retrospective study found high agreement in FVIII activity measurement between one-stage clot-based and chromogenic assays, suggesting reliability in clinical settings.

Key Points

  • Excellent agreement was observed between one-stage clot-based and chromogenic assays in measuring FVIII activity.
  • The mean FVIII activity measured by both assays was 1.2 IU/ml, with a minimal bias of -0.0034 detected in Bland-Altman analysis.
  • The study analyzed 3623 samples from various haematologic conditions over a nine-year period, indicating robustness in results.
  • Samples with FVIII activity less than 0.4 IU/ml showed a 21.1% discrepancy, highlighting the importance of careful interpretation in low activity cases.

Cite This Study

Buraidi et al. (2025) studied this question.

synapsesocial.com/papers/68c6df6de03a6c7bdc170f2ahttps://doi.org/10.1097/mbc.0000000000001384
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Clinical utility and impact of the use of the chromogenic vs one‐stage factor activity assays in haemophilia A and B2019 · 40 citations
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  4. 4Haemophilia A mutations in patients with non-severe phenotype associated with a discrepancy between one-stage and chromogenic factor VIII activity assays2014 · 66 citations
  5. 5Genetics and Hemostatic Potential in Persons with Mild to Moderate Hemophilia A with a Discrepancy between One-Stage and Chromogenic FVIII Assays2020 · 7 citations