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September 12, 2025AnimalsOpen Access

Cytogenetic Screening on Mediterranean Italian River Buffalo Males Intended for Reproduction and Females with Fertility Issues—A Pilot Study

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All males exhibited a normal karyotype, while one had an abnormal X-chromosome, indicating genetic variations.
Seven females were found affected by freemartinism, showing XX/XY leukocyte chimerism, leading to their removal.
Karyotype analysis was conducted using CBA- and RBA-banding techniques to investigate chromosomal health.
This investigation supports enhanced genetics in the Mediterranean Italian River Buffalo for artificial insemination.

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Pilot study reports karyotype analysis results in 159 males and 30 females, indicating cytogenetic issues affecting fertility.

Perucatti et al. (2025) studied this question.

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