Allele frequency of reserve protein loci in ukrainian breeding varieties

Aim. To determine the allele frequency of reserve protein loci in varieties of Ukrainian breeding developed by two different breeding centres: The Institute of Plant Physiology and Genetics of the National Academy of Sciences of Ukraine (IFRG of the National Academy of Sciences of Ukraine) and the Selection and Genetic Institute - National Centre for Seed Science and Variety Studies of the National Academy of Agrarian Sciences of Ukraine (SGI − NCSC of NAAS of Ukraine). Methods. The material for the study was 50 varieties of wheat of IFRG of NAS of Ukraine and 20 varieties of SGI − NCSC of NAAS of Ukraine. High molecular weight glutenin subunits were analysed by electrophoresis in the presence of sodium dodecyl sulfate (SDS) according to the Lemmley method. PCR with primers to the microsatellite locus SCM9 and electrophoresis of the storage proteins were used to determine the translocation. Results. The composition and allele frequencies of loci Gli-A1, Gli-B1, Gli-D1, Glu-A1, Glu-B1, Glu-D1 in the samples of varieties of IFRG of NAS of Ukraine and SGI − NCSC of NAAS of Ukraine were determined. The alleles of gliadin and high molecular weight glutenin loci, which are characteristic of varieties of a particular breeding centre, were identified. Conclusions. Based on the study of alleles of reserve protein loci, it was shown that groups of varieties developed in different breeding centres are characterised by a certain set of alleles. The most frequent alleles among wheat varieties developed by IFRG of NAS of Ukraine were those of high molecular weight glutenins Glu-A1a, Glu-B1c, Glu-D1d and gliadins Gli-A1b, Gli-B1b, Gli-D1b, and among Odesa varieties - alleles of Glu-A1b, Glu-B1u, Glu-D1d and Gli-A1b, Gli-B1b, Gli-D1g. It was found that almost 50 % of varieties of IFRG of NAS of Ukraine have wheat-rye translocations 1AL.1RS and 1BL.1RS.