Simultaneous fMRI and triple-sensor fiber photometry reveal the neurochemical correlates of bilateral striatal connectivity

Motivation: Robust correlations between bilateral striatal connectivity have been broadly reported; however, direct anatomical projections across hemispheres in this region are absent. The circuit mechanism remains unknown. Goal(s): We propose to elucidate the circuit origins of interhemispheric striatal resting-state fMRI connectivity using simultaneous fMRI and spectrally resolved triple-sensor fiber photometry. Approach: We measured neuronal calcium, dopamine, and glutamate dynamics in the striatum during fMRI. Approach: We measured neuronal calcium, dopamine, and glutamate dynamics in the striatum during fMRI. Results: Dopamine shows the most robust correlation between striatal regions. Impact: This work provides evidence-based interpretation of the rs-fMRI connectivity between striatum of the two hemispheres, which is crucial for motor control, cognition, motivation behavior, and several neurological disorders.