Seamless integration of fMRI (EPI, ZTE) and wild-field optical imaging in mice

Motivation: The correspondence of neural and fMRI FC is unknown but has seldom been examined due to challenge of simultaneous neuronal imaging during fMRI. Goal(s): Evaluating the feasibility of optical neuronal imaging during fMRI on FC spatial and temporal patterns. Approach: By a systemic integration of wide-field optical imaging and fMRI, we compared neuronal voltage signals or calcium signals in functional connectivity (FC) with simultaneous fMRI, EPI or ZTE in mice. Results: We demonstrated the method feasibility and different detection sensitivity on FC temporal and spatial patterns among modalities. Impact: Our results demonstrate the feasibility of the seamless integration of fMRI and optical imaging to the era of multi-modality neuroimaging.