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September 17, 2025Frontiers in Medical EngineeringOpen Access

Confocal fluorescence microscopy for real-time breast cancer diagnosis: current advances and future perspectives

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Authors

KVK. VyasAEAhmed EzzatNHNicholas H. G. Holford

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Overview

This review reveals technological progress in confocal fluorescence microscopy systems for breast cancer, suggesting improved diagnostic accuracy and surgical workflow.

Key Points

  • Fibre-based confocal fluorescence microscopy systems offer real-time imaging accuracy up to 94%, enhancing breast cancer diagnosis.
  • Bench-top systems showed high-resolution imaging accuracy ranging from 83% to 99.6%, but their complexity limits intra-operative use.
  • Integrating artificial intelligence and robotics into confocal fluorescence microscopy may significantly improve surgical decision-making.
  • Current technological developments like the Histolog confocal microscopy can enhance margin assessments, identifying missed tumors in a substantial number of cases.

Cite This Study

Vyas et al. (2025) studied this question.

synapsesocial.com/papers/68d4605131b076d99fa5fa6ehttps://doi.org/10.3389/fmede.2025.1607453
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  3. 3Towards a New Standard: Prospective Validation of Ex Vivo Fusion Confocal Microscopy for Intraoperative Margin Assessment in Breast-Conserving Cancer Surgery2025 · 2 citations
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  5. 5Editorial: Applications of fluorescence in surgery and diagnostics, volume II: evolution and breakthroughs2026