Paraoxonase Gene Polymorphism Analysis in Pediatric Diabetic Patients

Objective: Identifying gene polymorphisms linked to childhood-onset diabetes mellitus (DM), a multifactorial disease, will illuminate its genetic underpinnings. This study aimed to explore the association between childhood DM and the polymorphisms Paraoxonase1 (PON1) 55 and PON1 192. Method: A total of 129 children who applied to our Child Health and Diseases Endocrinology outpatient clinic participated in this prospective research. The study included 75 children with diabetes, aged 3–15 years (Patient Group-PG), and 54 healthy children (Control Group-CG) as a control. Genotyping of PON1 55 and PON1 192 polymorphisms was performed in study patients using Polymerase Chain Reaction (PCR) and RFLP methods following DNA isolation. Results: Of 54 CG, 21 (38.9%) displayed the homozygous normal (LL) PON1 55 genotypes. The PG contained 25 (33.3%) homozygous normal patients out of 75. In the CG, 25 participants (46.3%) and 39 participants in the PG (52.0%) had the heterozygous (LM) genotype. The homozygous mutant (MM) genotype was observed in 8 (14.8%) of CG and 11 (14.7%) of PG. The CG showed 67 L alleles, representing 62.0% of the total alleles. On the other hand, the number was 89 (59.3%) in the PG. The two groups showed no statistically significant differences in gene polymorphisms, allele frequencies, and M and R allele distribution. Conclusion: No significant relationship was observed between PON1 55 and PON1 192 gene polymorphisms and DM in our study.