Genetic characteristics of the first human adenovirus genotype 55 isolate (Adenoviridae, Mastadenovirus) from Moscow.

The aim of this work was to identify HAdV isolated in Moscow in 2022 and conduct a comparative genomic study. Material and methods. HAdV-55 was isolated from a sample of a patient hospitalized with pneumonia and analyzed using restriction fragment length polymorphism analysis and whole genome sequencing. Bioinformatic comparative analysis was performed on a sample of 83 isolates. Results. The genome of HAdV-55 isolated for the first time in the Russian Federation (isolate SCV3008:Ad55) was deposited in GenBank (Accession Number PQ641625). Unique mutations in the SCV3008:Ad55 genome were identified, one of which resulted in a conservative T29A substitution in the penton that did not affect its functions. Phylogenetic analysis showed clustering of SCV3008:Ad55 with isolates of clade II, which included representatives of 7 countries on different continents, indicating a wide distribution of HAdV-55. Isolates from endemic regions of China and South Korea formed separate clades. The study of microsatellite length polymorphism in untranslated regions of the genome became an additional tool for distinguishing closely related genomes. Conclusion. The obtained genomic information laid the foundation for further monitoring of HAdV-55 in Russia and demonstrated the informativeness and significance of whole-genome studies for monitoring adenoviruses.