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This is a Quantitative Study study.

Chitosan from Rhizopus stolonifer: Process optimization, structural characterization and selective antimicrobial activity

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This study demonstrates chitosan's antimicrobial activity against Gram-positive bacteria using optimized extraction from Rhizopus stolonifer.

The study shows effective antimicrobial activity of chitosan against Gram-positive bacteria, particularly Bacillus cereus and Candida albicans.
Chitosan was extracted with a maximum yield of 81.4 mg and a deacetylation degree of 72.53%, confirmed by FTIR spectroscopy.
Optimal growth conditions for Rhizopus stolonifer included a pH of 6.0 and a temperature of 30°C, leading to significant biomass accumulation.
The focus on R. stolonifer highlights its potential as a sustainable source for high-quality chitosan with applications in agriculture and biomedicine.

Azeez et al. (2025) studied this question.

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This study demonstrates chitosan's antimicrobial activity against Gram-positive bacteria using optimized extraction from Rhizopus stolonifer.