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September 20, 2025CureusOpen Access

1,25-Dihydroxyvitamin D Testing in Clinical Practice: A Literature Review on Diagnostic Challenges Arising From Analytical Variability

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Analytical variability significantly impacts the measurement of 1,25-dihydroxyvitamin D in clinical practice, leading to diagnostic challenges.
The review discusses how different assays for measuring 1,25-dihydroxyvitamin D vary in performance, with limitations affecting clinical reliability.
Current assays reflect a range of analytical principles that exhibit both strengths and weaknesses in quantifying vitamin D metabolites.
The lack of a standardisation scheme complicates comparability among various assays for measuring 1,25-dihydroxyvitamin D concentrations.

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Literature review highlights challenges in measuring 1,25-dihydroxyvitamin D, suggesting a need for standardisation and improved assays.

Farag et al. (2025) studied this question.

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