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September 23, 2025Frontiers in Cellular and Infection MicrobiologyOpen Access

Label-free metabolic imaging of Pseudomonas aeruginosa infection using two-photon fluorescence lifetime imaging microscopy

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Authors

GSGreg A. SnyderUniversity of MaryTCTialfi Bergamin de CastroASAlison ScottUniversity of Maryland, Baltimore

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Implication

This study demonstrates label-free imaging of multidrug-resistant bacteria and infected cells, suggesting new methods for understanding microbial infection.

Key Points

  • The method identifies and quantifies metabolic differences in live bacteria using fluorescence lifetime imaging.
  • High-resolution imaging in live samples reveals metabolic changes related to Pseudomonas aeruginosa infection.
  • The combination of two-photon microscopy and phasor plot analysis enables sensitive and non-destructive imaging.
  • Real-time monitoring of NAD(P)H metabolism enhances understanding of microbial pathogenesis and therapy evaluation.

Cite This Study

Snyder et al. (2025) studied this question.

synapsesocial.com/papers/68d4724731b076d99fa6aad0https://doi.org/10.3389/fcimb.2025.1642009
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