The effect methylglyoxal on acute lung injury induced by influenza A(H1N1)PDM09 in mice

The aim of the article. To study of the effect of 2-oxopropanal (methylglyoxal) on virus-induced acute lung injury. Materials and methods. The study was performed on adult female outbred mice. Methylglyoxal administered subcutaneously at a dose of 50 mg/kg/day to mice for 14 days prior to infection. The pandemic influenza virus A(H1N1)pdm09 was used for modeling viral infection at a dose of 0.75 LD50. Hematology, pathomorphological and histological studies were performed on 4, 7 and 14 days post infection. Level of lung injury was performed by semi-quantitative method. Results. Methylglyoxal induced 2-fold increase of mortality and lung lesion area (р 0.05). The structural changes in lung tissue had inflammatory character. These changes had progressive character. The ratio of neutrophiles/lymphocytes was increased by 2.5 times on average in infected animals (р 0.05 compared to intact animals). Conclusion. Methylglyoxal aggravated acute lung injury in mice by inducing structural changes in tissue and increased mortality level.