Genetic polymorphism within Chicken growth hormone and significant association of Perilipin gene with growth traits in Kadaknath chicken

The research work was planned to identify polymorphisms within Chicken growth hormone (cGH) and Perilipin (PLIN1) gene in Kadaknath chicken and test association of polymorphism with growth and carcass traits. Genomic DNA from 118 Kadaknath birds was estimated using DNA extraction kit. Restriction digestion of intron-3 region of cGH gene fragment was polymorphic with ‘G’ allele frequency as 0.85 and ‘A’ allele frequency as 0.15. Restriction digestion of 545 bp gene fragment of exon-6 region of PLIN1 gene using Tsp4CI enzyme, revealed polymorphism at PLIN1KC1-Tsp4CI locus with frequency of C1 allele as 0.65 and C2 allele as 0.35. The PLIN1 ‘C2C2’ genotype was associated with body weight at BW8, BW10 and BW12 weeks and ADG at 6-8 weeks and 10-12 weeks of age at PLIN1KC1- Tsp4CI PCR-RFLP locus in Kadaknath population. The 5′flanking region of PLIN1 using direct sequencing of birds revealed four SNPs. The study revealed polymorphisms in cGH and PLIN1 genes loci in experimental Kadaknath population. The identified association of PLIN1KC1-Tsp4CI with body weight and average daily gain may be used for selection of chicks for growth traits and for genetic improvement after validation in larger dataset.