This protocol standardizes iPSC culture to enhance viability and reduce variability, indicating robust applications in differentiation and gene editing.
To ensure consistent and high-quality maintenance of induced pluripotent stem cells (iPSCs) by implementing standardized protocols and adhering to good cell culture practices. This includes minimizing inter- and intra-batch variability, preventing microbial and cross-contamination, and optimizing culture conditions such as media composition, passaging frequency, and confluency thresholds to support reproducible and robust downstream applications including differentiation, gene editing, and transcriptomic profiling.
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Kim et al. (2025) studied this question.
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