What question did this study set out to answer?

To establish a standardized, cost-efficient protocol for the cryopreservation, banking, and quality assurance of human induced pluripotent stem cells in basic research.

September 21, 2020Current Protocols in Stem Cell BiologyOpen Access

Cell Banking of hiPSCs: A Practical Guide to Cryopreservation and Quality Control in Basic Research

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Authors

ASAya ShibamiyaUniversität Hamburg ESE. SchulzeUniversität Hamburg DKDana KraußMedical University of Vienna

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Implication

Protocol paper demonstrates cost-efficient cryopreservation and quality control in human induced pluripotent stem cells, highlighting strategies to improve experimental reproducibility.

Key Points

To establish a standardized, cost-efficient protocol for the cryopreservation, banking, and quality assurance of human induced pluripotent stem cells in basic research.
Outlines two-tiered cell banking protocols to generate master and working cell banks through controlled cell expansion and cryopreservation.
Implements quality-assurance workflows comprising thawing recovery, sterility screening, pluripotency assessment via flow cytometry, spontaneous differentiation via qRT-PCR, and identity verification via short tandem repeat analysis.
Provides practical, low-cost procedures to mitigate phenotypic and functional deterioration observed across prolonged hiPSC culture passages.
Establishes standardized benchmarks for post-thaw cell viability, microbial sterility, multi-lineage differentiation potency, and genomic integrity to ensure experimental reproducibility.

Cite This Study

Shibamiya et al. (2020) studied this question.

synapsesocial.com/papers/6a8668b4e7c8470ab14cb0d4 https://doi.org/10.1002/cpsc.127

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Implication

Protocol paper demonstrates cost-efficient cryopreservation and quality control in human induced pluripotent stem cells, highlighting strategies to improve experimental reproducibility.

Cell Banking of hiPSCs: A Practical Guide to Cryopreservation and Quality Control in Basic Research

Discussion

Member takes

Implication

Key Points

Cite This Study

Cell Banking of hiPSCs: A Practical Guide to Cryopreservation and Quality Control in Basic Research

Discussion

Member takes

Implication

Key Points

Cite This Study

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