Diuretic Activity of Benzoylteophylline

Introduction. The study is devoted to the study of the diuretic activity of benzoyl theophylline, a derivative of theophylline with high calculated diuretic activity, in an animal experiment, the prediction of which was made using the PASS Online service. Objective. The aim of the study was to study the effect of benzoyl theophylline on the excretory function of the kidneys of rats. Materials and Methods. The experiments were conducted on 18 male stock Wistar rats aged 2-3 months and weighing 250-300 g, which were divided into 2 groups: the control (intact rats) and the benzoylteophylline group. The duration of the experiment was 12 days, of which, in the first 10 days, benzoyl theophylline was administered daily intragastrically at a dose of 15 mg/kg, after which the administration of the substance was discontinued in the final 2 days. Daily urine was collected and the renal excretion of Na+, K+, and creatinine ions was measured. Statistical processing of the obtained data was carried out using nonparametric methods of statistical analysis. Results. It was found that as a result of 10-day administration of benzoyl theophylline, a consistent increase in daily diuresis in rats was observed by 4.9 times, from 5.5 (2.5;9.7) ml/day at baseline to 26.8 (9.0;30.7) ml/day on day 10 (p=0.008). As a result, the level of diuresis in the benzoyl theophylline group was 5.7 times higher than in intact rats (p=0.008). In the control group, the level of diuresis did not change throughout the experiment. Urinary excretion of sodium and potassium in the benzoyl theophylline group increased 5.8-fold (p=0.008) and 8.8-fold (p=0.008), respectively, relative to baseline levels during the experiment. In the control group, the level of renal excretion of sodium and potassium remained stable throughout the experiment. Renal creatinine excretion in all three experimental groups remained unchanged throughout the experiment. Conclusion. As a result of the study, the presence of pronounced diuretic activity in benzoylteophylline was revealed. Further research will focus on a deeper study of the pharmacokinetics and pharmacodynamics of benzoylteophylline in order to create a new diuretic drug based on it.