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September 28, 2025Open Access Health Scientific Journal

Validation of the UV-Vis Spectrophotometric Method for the Determination of Ascorbic Acid Content in Beverage Preparations Based on a Standard Vitamin C Calibration Curve

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Authors

DADevita Riafinola AndariritInstitut Ilmu Kesehatan STRADA IndonesiaSPSri Rahayu Dwi PurnaningtyasInstitut Ilmu Kesehatan STRADA IndonesiaAWArif Kurnia WijayantoJapan Society for the Promotion of Science

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Implication

Analytical method reveals ascorbic acid levels in beverages, indicating accuracy in measurements.

Key Points

  • Ascorbic acid levels were measured with high precision and accuracy using a UV-Vis spectrophotometer.
  • The linear regression analysis yielded a strong correlation with an r2 value of 0.995.
  • The method's limit of detection and quantitation were found to be 0.429 ppm and 1.3 ppm, respectively.
  • Results confirmed that vitamin C content in beverages complied with Pharmacopoeia standards.

Cite This Study

Andaririt et al. (2025) studied this question.

synapsesocial.com/papers/68d9052141e1c178a14f50f0https://doi.org/10.55700/oahsj.v6i2.101
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Also Consider

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  3. 3Development and validation of an LC-MS/MS method for the analysis of total ascorbic acid capacity in plasma2026 · 2 citations
  4. 4Spectrophotometric Determination of L-Ascorbic Acid in Pharmaceuticals based on Its Oxidation by Potassium Peroxydisulfate in the Presence of Ag(I) as a Catalyst2024
  5. 5B-174 Quantification of vitamin C in serum as a biomarker using the Laser Diode Thermal Desorption technique “LDTD”/Mass Spectrometry (Luxon)2024