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March 25, 2026Journal of Chromatography BOpen Access

Development and validation of an LC-MS/MS method for the analysis of total ascorbic acid capacity in plasma

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Authors

DKDylan S. KellyCLClaude P. van der LeyRBRon Booij

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Overview

Validation of an LC-MS/MS method reveals 10% vitamin C deficiency and 20% hypovitaminosis in patients, suggesting increased screening is necessary.

Key Points

  • The aim is to develop and validate a method for accurately quantifying total ascorbic acid levels in plasma.
  • Developed a robust LC-MS/MS method for quantifying ascorbic acid in plasma.
  • Used EDTA blood samples processed within two hours to preserve vitamin C levels.
  • Dehydroascorbic acid was reduced to ascorbic acid using TCEP before analysis.
  • Assessed 547 patient samples to determine vitamin C deficiency prevalence.
  • Achieved a limit of quantification of 1.1 μmol/L, suitable for detecting deficiency.
  • Found that 10% of patients were vitamin C deficient and 20% had hypovitaminosis.
  • Males showed approximately 11 μmol/L lower vitamin C levels than females, independent of age.

Cite This Study

Kelly et al. (2026) studied this question.

synapsesocial.com/papers/69c37aa8b34aaaeb1a67c8cdhttps://doi.org/10.1016/j.jchromb.2026.125023
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