What question did this study set out to answer?

This study aims to validate an automated extraction protocol combined with LC-MS for quantifying plasma vitamins A and E.

Clinical validation of an automated extraction protocol and liquid chromatography single quadrupole mass spectrometry (LC-MS) method for plasma vitamins A and E analysis

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Randomized trial evaluates LC-MS for plasma vitamin analysis, showing high accuracy and efficiency.

This study aims to validate an automated extraction protocol combined with LC-MS for quantifying plasma vitamins A and E.
Utilized Oasis PRiME HLB µElution Plates for automated extraction on a Tecan Liquid-Handling Platform.
Analyzed plasma samples using LC-MS, comparing with the HPLC-UV method for accuracy.
Evaluated 70 patient specimens, including controls and external quality assessment materials.
Inter-assay imprecision was acceptable at CV≤5 %.
Bias for vitamin A was 5.67 % and for vitamin E was -0.15 %.
Mean bias for both vitamins using EQA materials was <4 %.

Hellier et al. (2026) studied this question.

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